HPLC Applikation

Suitable for Your Application

Highly versatile, customizable and modular

From liquid chromatography processes to rock analyses in mineral oil production and all the way to industrial coating processes of varnishes and lubricants – neMESYS syringe pumps have more to offer and are therefore used in a wide variety of industries.

The highly precise and pulsation-free dosing of tons of liquid per year or just nano-liters per hour, as well as the modular and highly flexible design of CETONI systems are only two of their most prominent characteristics. Following, you will learn more about how our systems are used in their various applications and which of their properties are required for this. 

Chemistry

Microfluidics in Chemistry

Microfluidics for Analytical Processes

Rapid advances in micro-fluidics led to the development of highly efficient chemical analytic processes. The miniaturization of fluid analysis makes it possible to work effectively with small reaction volumes and therefore places high demands on the hard and software components being used, particularly regarding precision and versatility.

Fluid Chromatography

Fluid chromatography processes, such as HPLC, require continuous fluid streams without pulsation, as well as media-resistant systems that are able to provide high pressure and can be cleaned quickly and easily. The highly flexible syringe pump systems of the neMESYS range, fulfill these requirements and are therefore the ideal alternative to conventional HPLC-pumps. Thanks to its modular design, CETONI offers an all-encompassing laboratory system that can be expanded easily, including automated sampling or fraction collection with the rotAXYS positioning system or through collaboration with laboratory robots, high-precision fluid transport using our neMESYS syringe pumps, or analysis functions with incorporated spectrometers.

Requirements, such as the fast and hard adjustment of solvent ratios, medium change or the ability to transport larger volumes do not pose a problem for the dosing system. Beyond that, the analysis process can be easily automated, using our powerful Qmix-Elements control software.

Darstellung HPLC

Flow Cytometry

Flow cytometry is a powerful analytic method for the precise determination and characterization of particles, such as cells, spores and blood samples. Depending on their application, these analytic processes often happen at moderate system pressure levels. An essential requirement for the success of these sensitive processes is a highly continuous stream of analytes, such as individual cells, for example.

The pulsation-free character and the superior precision of neMESYS syringe pump drives is therefore ideal for analytic processes in the sub-microliter range. The combination and synchronization with periphery devices, such as laboratory cameras, can be interesting for the continuous detection of cells in a fluid stream. A space saving, modular and expandable system is a big asset in this case.
Of course, the increasing number of devices and the increasing complexity of applications also leads to higher demands regarding the operation and synchronization of all components involved. Our powerful and user-friendly QmixElements software is the answer to all of your automation needs and gives you more room for creativity.

Setup

The appropriate devices for this application

Low Pressure Syringe Pump neMESYS 290N

Low Pressure Syringe Pump neMESYS 290N

Pumps

High Pressure Syringe Pump neMESYS 2600N

High Pressure Syringe Pump neMESYS 2600N

Pumps

3/4-Way Contiflow Valve (10 bar max.)

3/4-Way Contiflow Valve (10 bar max.)

Valves

360° Positioning System rotAXYS360

360° Positioning System rotAXYS360

Sample Handling Modules

Geology and Petrochemistry

Microfluidic applications

Rock Analysis with Microfluidic Methods

EOR and pVT – High Pressure Syringe Pumps used in Crude Oil Research

The global demand for crude oil increases every year. At the same time, we know that crude oil is a finite and non-renewable resource. Therefore, the search for methods enabling an improved exploitation of existing reservoirs and the related research has started some years ago. After all, the “black gold” is rarely found in underground caverns, but embedded in rocks. The physical and chemical properties of these rocks and the oil contained in them vary from location to location. In order to extract the oil, bore holes are filled with gas or liquids. Chemical (tensides) and physical methods (temperature), and even micro-organisms are employed in this process. The goal is to lower the oil’s viscosity and the surface tension between oil and rock, and doing it in a targeted manner, in order to extract as much oil as possible. All of these methods are referred to as tertiary exploration or enhanced oil recovery (EOR). Since this type of research requires a precise simulation of storage and extraction conditions, the use of CETONI high-pressure syringe pumps makes sense. Many companies and institutes all over the world already use CETONI equipment in this field and have achieved and published promising research results.

Hochdruckspritzenpumpe

Once the oil is at the surface, it becomes clear that there are different types of oil. Due to different compositions and rheological properties, products from different reservoirs have to be analyzed and characterized at regular intervals. The crude oil’s composition decides which products and which quantities can be made from a certain quantity of crude (e.g. a barrel). This has an effect on the price that can be achieved with this product and the profitability of the respective bore hole.

The rheological properties, meaning the deformation and flow properties of the crude oil, influence the extraction speed and the maximum degree of exploitation for a particular reservoir. This, in turn, is a critical factor in the profitability of oil extraction as well as the availability of this desperately needed resource. An important method for both – composition and rheological properties – is the pVT analysis. In this process, a specific amount of the sample at a specific temperature is pushed through a capillary of defined diameter and length. As the liquid passes through the capillary, the pressure loss is measures. If this measurement is taken at various temperatures, one receives a whole grid of the sample’s important physical properties. The accuracy of the measured data is governed by the parameters set during the analysis.

CETONI’s high-pressure syringe pumps are the preferred equipment used for these measurements with pVT cells, since they are able to transport liquids precisely and without pulsation, even at pressure values exceeding several hundred bar. In addition, they can easily be expanded with features like pressure measurement or a heating system.

Hochdruckspritzenpumpe

The appropriate devices for this application

Ultra High Pressure Syringe Pump neMESYS XL 7000N

Ultra High Pressure Syringe Pump neMESYS XL 7000N

Pumps

Pneumatic High-Pressure Valves

Pneumatic High-Pressure Valves

Valves

Temperature Control Module Qmix TC2

Temperature Control Module Qmix TC2

Tempering / Reaction Modules

External Pressure Sensors

External Pressure Sensors

I/O Components

Industrial Processes

Applications in Industrial Processes

Precision Dosing

The use of micro-fluidic processes is not limited to research labs. Industrial companies are already successfully using CETONI systems for the precision dosing of fluids. The bandwidth of application is enormous and ranges from model solutions to large-scale and automated systems.

Varnishes, lubricants and adhesives – Precision Dosing in industrial Applications

The application of finishes requires a jerk-free media supply system. Our high-precision syringe pumps are capable of dosing without pulsation and at high precision down into the nano-liter range, therefore providing a homogenous coating process. A media supply that is interruption-free and independent from the syringe volume is made possible by combining our special valves with our innovative QmixElements control and automation software, which smoothly blends the operation of alternating syringe pumps during continuous media transport.

Thanks to its modular design and the available interfaces, the dosing system can be easily expanded and integrated into existing coating systems. In addition, its operation is simple and allows for efficient processes, thanks to the ability to exchange syringes very fast instead of employing complex rinsing and cleaning procedures.

Darstellung industrielle Beschichtung

Flow Chemistry

Flow chemistry is becoming increasingly interesting with regard to the implementation of industrial processes. Requiring only small quantities of poisonous or highly sensitive substances makes an important contribution toward achieving improved process safety and control. This simplifies the control of endothermic and exothermic processes as well as elevating temperatures beyond the boiling point with regard to critical reactions. The miniaturization of processes increases efficiency, while lowering costs at the same time. Even scaling up a proven reaction becomes quick and easy. 

Particle Synthesis

Particle synthesis requires a setup providing extremely precise fluid dosing at very continuous flowrates. A media supply that is uninterruptable and independent from the syringe volume (continuous flow) is made possible by our innovative QmixElements control and automation software. By using an intelligent algorithm, it ensures the smooth blending of alternating syringe pumps during continuous media transport, to a point where it becomes practically unnoticeable. Thanks to the ability to choose from various different material combinations with regard to the medium-affected components, the fluid system is resistant to many different chemicals. Also, it makes no difference whether the system is operated under normal atmospheric conditions or high pressure. The neMESYS technology fulfills both requirements with excellent results.

Apart from compact setups, our modules can also be used to realize highly complex systems, that feature pressure and temperature control or could be fitted with sample handling components, such as our rotAXYS system or additional analysis modules. In addition, they can be automated using our comprehensive QmixElements controlling software.

Darstellung Partikelsynthese

The appropriate devices for this application

Mid Pressure Syringe Pump neMESYS 1000N

Mid Pressure Syringe Pump neMESYS 1000N

Pumps

Ultra High Pressure Syringe Pump neMESYS XL 7000N

Ultra High Pressure Syringe Pump neMESYS XL 7000N

Pumps

Heated Syringes

Heated Syringes

Syringes

Modular I/O Module Qmix I/O-B

Modular I/O Module Qmix I/O-B

I/O Modules

Biology

Microfluidics in biology

Bio-Printing

For successful printing of biologic materials, such as cells and tissue with their highly complex structure, precise fluid dosing is just as important as a continuous supply of material to the printing head.
Only in this way is it possible to create homogenous layers with a high degree of reproducibility. Thanks to their precision and smooth delivery, our neMESYS syringe pumps are perfectly suited for applications in this area. The sophisticated syringe holders are easy to clean and can even be used with sterilized glass syringes or single-use syringes.

The QmixElements software lets you control even complex setups for the combination of different media and different module units and it lets you automate defined processes very easily.

Spritzenpumpensystem

Analysis of soil outcrops in the compartmentalized flow

The so-called segmented flow is one of the wonderful tools in the microfluidic toolbox. This flow is generated in micro-reactors featuring channels with a diameter of 100 to 200 µm. A polar liquid (water) is introduced to an intersection point of a nonpolar carrier flow (oil). Since the two liquids will not mix, individual water droplets will be torn away and join the oil flow. The droplet sizes are very defined and constant and they happen at defined intervals, while being separated from each other by the oil between them. Large amounts of these droplets can be created in a very short time. If the soil concentration in the water is correct, each droplet contains just one individual or very few organisms, existing in their own habitat, where they can procreate and form cultures. Arranged on a coil of narrow tubing, similar to beads on a pearl necklace, they can now be taken to an incubator offering the appropriate growing environment.

Generating such small, defined and segmented flows requires highly precise, pulsation-free and controllable pumps, such as the neMESYS 290N low-pressure pump made by CETONI. Thanks to its excellent dosing properties, substances, such as nutrients, can be dosed into the individual droplets. Also, it is possible to detect clouding and separate promising droplets. The Qmix Elements software allows users to operate the system comfortably and intuitively.

Setup

The appropriate devices for this application

Low Pressure Syringe Pump neMESYS 290N

Low Pressure Syringe Pump neMESYS 290N

Pumps

Syringe Stirrer neMIX 50

Syringe Stirrer neMIX 50

Stirring Systems

Compact Positioning System rotAXYS

Compact Positioning System rotAXYS

Sample Handling Modules

Spectrometer Module Qmix Lambda

Spectrometer Module Qmix Lambda

Detection Modules